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This ectopic targeting of Bub1p, Bub3p and Mad3p to telomeres had no consequence on cell cycle progression.
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Functional consequences on cell cycle and cell motility were determined by fluorescence activated cell sorting (FACS) analysis and cell migration assay respectively.
We report here that the mTORC1-mediated consequences on cell cycle and cell size are separable and do not involve effects on mTORC2 activity.
Making use of non-transformed, non-immortalized primary human fibroblasts we found that (i) the mTORC1-mediated consequences on cell cycle and cell size are separable and do not involve effects on mTORC2 activity; (ii) In addition, we report here for the first time that mTORC2 is a potent regulator of mammalian cell size and cell cycle via a mechanism involving Akt/TSC2/Rheb.
The reduction of BRCA1 foci did not appear to be a consequence of changes in cell cycle, as the silencing of Cav-1 had no effect on cell cycle distribution (Fig. 6).
The flow cytometry test of cell cycle showed RCAN1 had no effect on cell cycle.
The large nuclear size of LmnaDhe/+ fibroblasts suggested that the cells might be aneuploid, which is often a consequence of cell cycle defects [24], [39] [41].
Apoptosis induction is often a consequence of cell cycle disturbances.
Chronic administration of gefitinib reduced intestinal tumourigenesis as a consequence of cell cycle inhibition.
The decreased expression of them might be a consequence of cell cycle arrest produced after 17AAG.
Apoptosis was also increased that might be the consequence of cell cycle deregulation and mitotic catastrophe.
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