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The difference in altitude between historical and recent records might be a consequence of sampling bias in historical times.
This may reflect the conservative parameters of our clustering methods (to be clustered, reads must possess 96% identity across 100 or more nucleotides), but also is likely a consequence of sampling bias resulting from small sample size (small numbers of clones sequenced from each library).
Although it is possible that the decreased frequency of observed heterozygotes is a consequence of sampling bias in this small population, it is also possible that for some markers where the size differences between alleles are small, HPLC may not be able to resolve the individual alleles.
An obvious consequence of sampling from clinical settings was that cases of headache on ≥15 days/month were very many (35.6%), even though 33 of the accompanying persons did not have headache at all.
In addition, Fig. 1 shows that the difference between the lowest- and the highest-SES groups and the difference between the medium- and the highest-SES groups tend to expand as age increases; however, this may be a consequence of sampling error.
The confidence intervals around the point estimate also allow the distinction of discrepancies in W values that are due to differences in the pattern of diversity of a given microbial population or that are only a consequence of sampling variability.
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Our value here is a bit higher, but this may be a consequence of samples of different size, geometry and purity used here, as well as a difference in measuring speed.
STRUCTURE detected additional subpopulations within these groups as a consequence of sample relatedness.
In addition to alterations in elemental distribution as a consequence of sample preparation, chemical speciation may also change.
We cannot rule out the possibility that the size difference is a consequence of sample fixation for the TEM studies.
Additional experiments are being conducted to probe whether the observed lactone profile has biological significance or is merely the consequence of sample preparation or LC conditions.
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