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The approach to defining consensus reference intervals is complex.
The genotype 1b population consensus reference sequence was generated from all 126 full-length ORFs from different patients in the databases in January, 2006.
We first compared the number of variations in the SVR or NR sequences relative to a genotype 1a or 1b population consensus reference sequence.
We first aligned the 1a and 1b sequences and generated a neighbor-joining tree, using a different subtype consensus reference as an out-group.
The genotype 1a consensus reference sequence was derived from all 12 full-length ORFs in the Los Alamos [35] and European [36] HCV databases in April, 2005, plus five 1a ORFs we sequenced from non-Virahep-C cohorts.
While the CLSI guidelines do consider interval variability to some degree, the significance of creating a consensus reference interval without a direct comparison of the upper and lower limits is unclear.
Similar(28)
All sequences were individually analyzed for similarity with consensus references by SimPlot analysis (Simplot 2.5 by Stuart Ray,21 http://sray.med.som.jhmi.edu/RaySoft/SimPlot/).edu/RaySoft/SimPlot/
In order to obtain the highest possible quality and resolution, reference-based iterative refinement was performed using the consensus references for these conformations on the respective data subsets once model independence was confirmed.
The product was sequenced and the results were compared to the consensus B reference sequence using HIVdb software (Stanford HIV Drug Resistance Database, http://hivdb.stanford.edu) to detect drug-resistance mutations.
Because there is no consensus on reference values of ODs to classify viral load, they were categorised in quintiles.
However there seems to be no consensus on reference genes for gene expression studies in stomach cancer.
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