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Identification of a putative cholesterol recognition/interaction amino acid sequence and consensus pattern.
Strictly conserved residues among all IPCT/DIPPS sequences are displayed in bold, and from the consensus pattern of CDP-alcohol phosphatidyltransferase family are enclosed in a box.
A putative TcAAD cDNA clone was identified on the basis of the consensus pattern and sequence homology to the published AADs in NCBI database.
A putative ClPDI cDNA clone was identified on the basis of the consensus pattern and sequence homology to other published PDIs in NCBI database.
Therefore, each character that is in conflict with the consensus pattern is designated as a homoplasy (i.e., rough texture) after the tree is already built (Fig. 1).
A putative TcTrx cDNA (640 bp) clone was identified on the basis of the consensus pattern and sequence homology to other published Trxs in NCBI database.
Sequences (one at a time) were entered in the restriction mapper site, results obtained were analyzed and consensus pattern was determined for each species depending upon its frequency of occurrence in the sequences.
The analysis of consensus pattern on C. glabrata promoters (−800 to −1) was performed using the Regulatory Sequence Analysis Tools (RSAT: http://rsat.ulb.ac.be/rsat/index.html) and implemented to the pattern discovery tool (oligo-analysis).
Their LRRs (hereafter GALA-LRR) have a specific consensus pattern with characteristic differences from the previously described consensus sequences of LRR subfamilies, especially from the known bacterial LRR subfamilies.
Due to the variations in the locations and lengths of NFRs, most of them are expected to be longer than their consensus pattern derived by curve averaging (c.f. aforementioned results of [5], [6]).
Since this only identified a motif in one quarter of the introns, we used the loose consensus pattern RYURAY (seen in the motifs found by AlignACE) and picked the 3'-most instance in each intron.
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