Sentence examples for consecutive washing from inspiring English sources

Cation exchange resin DOWEX 50 H+ was regenerated by consecutive washing with HCl (3 M), water and dry MeOH.

Unbound substances are removed in two consecutive washing steps.

A series of consecutive washing steps using PBS buffer rendered a precipitate containing the protein of interest, in its insoluble form, with purity higher than 90%.

A series of consecutive washing steps using PBS buffer solution rendered a precipitate containing the protein of interest in the insoluble fraction with a purity of above 90%.

Release of the PDMS from the mold was achieved under liquid conditions with ethanol as a lubricant, with consecutive washing in distilled water.

After four consecutive washing steps specifically bound proteins were eluted with SDS containing buffer from the magnetic beads and separated by SDS gel electrophoresis.

Bacterial endotoxin was removed by consecutive wash steps of the protein with Triton X-11424.

Excess avidin was removed by three consecutive wash cycles using the same citrate buffer.

After three consecutive washes in wash buffer, cells were incubated for 45 min with secondary antibodies (Alexa Fluor Donkey-anti-mouse and Alexa Fluor Donkey-antInvitrogenInvitogethertogether with Hoechst 33342 (Invitrogen).

Unbound proteins were washed out by 4 consecutive washes with RIPA buffer and 2 washes with kinase buffer (mM HEPES (pH 7.5), 15 mM MgCl2, 5 mM EGTA, 1 mM DTT) [85], [86].

Three consecutive washes were performed with the binding buffer to remove the BSA followed by the elution of nickel-bound P22sTsp with 100 µl of 50 mM Tris-HCl/500 mM NaCl/500 mM imidazole, pH 7.5.