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All primers for the single/low-copy sequences were designed using the software PRIMER 5.0 with the criteria that the primer length should be 20 nucleotides, with a 40 60% GC content, no secondary structure, and no consecutive tracts of the same nucleotide.
Candidate primer sequences were subsequently filtered through following parameters: length range from 18 to 24 bp, devoid of consecutive tracts of single nucleotide, GC content around 50% with a theoretical Tm approximately 60°C, and perfect ending with G- or C-rich at the 3′ end.
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The study included consecutive respiratory tract samples from children under the age of 17 years, who were hospitalized at the Department of Pediatrics, University Hospital Bern, between May 1st 1998 and April 30th 2010.
The prevalence of class 1 integrons in E. coli was 49% in selected non-outbreak isolates from hospitalized patients from 2002 to 2004 in our study, 52% in consecutive urinary tract isolates in 2001 in Sweden (10 ), and 15% in isolates from blood in hospitals in Sweden in 1998 and 1999 (11 ).
Validation studies across 190 dried blood spots demonstrated 100% sensitivity and a positive predictive value of 100% for single-nucleotide variants and insertions and deletions and complete concordance across the polymorphic poly-TG and consecutive poly-T tracts.
ABDC inhalation therapy is discontinued after two consecutive daily respiratory tract cultures or after detubation of the patient.
We then examined the coding sequence for each homo-AA tract to determine if it is encoded by a TNR: a homo-AA tract is considered TNR-encoded if at least seven consecutive residues of the tract were encoded by the same codon.
To ensure that locally low SNP density does not increase the length of an ROH, a required minimum density was set at 50 kb/SNP and the maximum gap between two consecutive SNPs in a tract was set at 100 kb.
Design, patients and methods fifty-eight iliac tracts in 53 consecutive patients with symptomatic arterial obstructive disease of the legs with an isolated stenosis with PSV ratio between 1.5 and 3.5 were studied prospectively.
We studied the activity of combined oxaliplatin and fluorouracil-leucovorin in 16 consecutive patients with advanced biliary tract adenocarcinomas.
We enrolled 1,359 consecutive patients with lower respiratory tract infections admitted to emergency departments of six tertiary-care hospitals in Switzerland.
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