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Consecutive induction of cellular activation and cell death is a well known phenomenon in leukocytes and termed activation induced cell death (AICD) [35].
After the consecutive induction of the N-terminal part (H6-GB1-NpuDnaE-IntN123), a large amount of the ligated product (H6-GB1-GB1) was accumulaned (lane 3 and 4, Figure 2b).
The batch cultivation was used to harvest biomass for consecutive induction by any of the different oligosaccharides at the final concentration of 200 mg L−1.
Such altered induction properties now enable the consecutive induction of coexpressed proteins such as chaperons and the therapeutic protein of interest.
Taken together, these data suggest that a consecutive induction of necroptosis and apoptosis represents a more efficient strategy for the elimination of cancer cells than a single induction of either mode of RCD.
In order to test the antitumor effect with this system, nude mice bearing breast cancer were treated with GCV followed by rAAV intratumoral injection and consecutive induction by Dox.
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The study subjects are female and male Karakal recruits from three consecutive inductions (September 2004 through July 2005).
For example, in the study cited above, in which participants took part in consecutive rumination induction tasks, the researchers assessed mood pursuant to each rumination induction and then engaged all participants in a mood augmentation task at the end of the study [ 41].
The treatment plan consisted of one administration weekly, for 3 consecutive weeks (induction treatment), followed by a 5-week rest period.
All received intravesical instillation of BCG for 6 consecutive weeks (induction BCG scheme, iBCG) and 56.4% were submitted to maintenance BCG schedule (iBCG+maintenance protocol with two weekly instillations every 3 months during 2 years, mBCG).
In the study of Santoni et al. glioblastoma patients older than 65 years were retrospectively analyzed, receiving FTM every week for 3 consecutive weeks (induction phase) and then every 3 weeks (70 100 mg/m), as second-line treatment.
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