Sentence examples for consecutive hybridization from inspiring English sources

Linkers were ligated to one batch of each sample ("tester") and hybridized with an excess of the other sample ("driver") using two consecutive hybridization steps.

The authors performed 100 PCR reactions to produce biotinylated baits covering the mtDNA and performed two consecutive hybridizations in solution.

However, according to the specification of the fluorescence imaging system used, the "transgenic probe plus single chromosome-specific probe" approach may require many consecutive FISH hybridizations to establish eventually the chromosomal location of the transgenic insertion(s).

OP explants were cultured for 24 h, corresponding in time to approximately stage 20 embryos, and analyzed by in situ hybridization on consecutive cryo-sections for the expression of Notch1, Delta1 and Hes5.

For example, in the consecutive sampling of duplicate hybridizations and biological culture replicates, an average 6.8% and 5.0% of the samples failed the Kolmogorov-Smirnov test at the level of 0.05.

Finally the following two gene-wise filtering criteria were applied: i) the presence of at least 75% of the spots per cDNA (at least 9 spots per cDNA out of a possible maximum of 12 spots in the arrays with hybridizations of consecutive time points or 16 spots in the arrays including day 0 nurse and forager day 17 samples), and ii) the availability of measurements at all ages.

Agilent human lncRNA + mRNA Array V3.0 was hybridized in an Agilent Hybridization Oven overnight at 42°C and washed with two consecutive solutions (0.2% SDS, 2x SSC for 5 min at 42°C, and 0.2x SSC for 5 min at room temperature).

In addition, our earlier studies with consecutive samples for RNA in situ hybridization and immunohistochemistry on adrenal samples have revealed the reliability of the used anti-GATA-4 and anti-GATA-6 antibodies in human tissues [ 28].

We explored their presence in a 300-base pair DXZ4 window by a Northern hybridization-based scanning approach using consecutive oligonucleotide probes.

Consecutive sections were used for fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC).

EBER in situ hybridization has even been performed on consecutive frozen or paraffin sections from the same blocks with divergent results between different centres (Rome, Amsterdam and Vienna).