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A total 105 consecutive CC patients (93% participation) stages IA IIIA, who met the inclusion criteria, were enrolled to the study; 58 (55%) had been treated with RAD/CHEM (group 1) and 47 (45%) with RAD (group 2).
Fifty-nine comprisedmprised of 5 normal liver tissues and 54 consecutive CC specimens (21 well-differentiated, 12 moderately-differentiated and 21 poorly-differentiated), were included in the study.
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Fraction C2 (1.07 g) yielded chrysoeriol (5) (80.70 mg) after two consecutive CCs (1st CC: Si-gel, EtOAc hexane gradient; 2nd CC: Sephadex LH-20, MeOH), followed by recrystallisation from ethanol.
Rainey went on to win three consecutive 500 cc world championships for Roberts' team.
Suzuki riders went on to win the last four races, but Roberts had built up a sufficient point lead to hold on and clinch his third consecutive 500 cc world championship.
The study was based on historical data from 155 consecutive patients with CC [69 intrahepatic CC (ICC) and 86 extrahepatic CC (ECC)] referred to Sant'Orsola-Malpighi University Hospital between 2006 and 2010.
Fraction B3 (5.05 g) provided cappariloside B (2) (14.80 mg) after two consecutive Si-gel CCs (1st CC: EtOAc hexane gradient; 2nd CC: acetone hexane gradient), followed by prep-TLC (2% MeOH CH2Cl2 as the eluent) and recrystallisation from EtOAc hexane.
Fraction B5 (2.15 g) after two consecutive Si-gel CCs (1st CC: CH2Cl2 hexane and CH2Cl2 MeOH gradients; 2nd CC: EtOAc hexane, and MeOH EtOAc gradients) gave fractions D1 D7.
Interestingly, a large portion of cysteine residues of EDCRP is present in the form of consecutive cysteine residues (CC) and notably, CCs are arranged in a regular pattern not only in the central repetitive region but also in the terminal segments.
The present exploratory case control analysis was initially based on a consecutive series of CC cases seen in our center and on historical controls.
Of note, two consecutive cysteine residues (CC) are found at a periodicity of 8 11 residues (with exceptions) along the entire length of EDCRP proteins.
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