Sentence examples for consecutive amplification from inspiring English sources

The outer primers were designed to anneal at higher temperatures than the nested primers to prevent primer competition in consecutive amplification reactions.

Both phages GFP-N-λ and GFP-C-λ formed normal size plaques as compare to the wild-type λKM10 vector phage and stably expressed GFP even after several consecutive amplification cycles.

These intron-containing AAV Rep and Cap coding sequences were very stable in recombinant baculoviruses and showed no apparent loss of protein expression even after five consecutive amplifications of the plaque-purified recombinant baculoviruses.

One key difference of the protocol described in the Materials and Methods section that we used to generate gene expression profiles from low amounts of RNA is that we performed three consecutive linear amplification steps, whereas the standard Affymetrix protocol involves only one linear amplification step.

Thus, the molecules that were hybridized onto microarrays underwent three consecutive linear amplification steps.

To minimize the effects of PCR artifact in the groEL dataset (no variation was detected among MLST clones), each consecutive PCR amplification served as a "reconditioning PCR" [ 32], using a small aliquot from an initial PCR.

Barcoded amplicons were produced by two consecutive PCR amplifications, following the procedure described in the previous section.

The parameter γ defines the net cell amplification between consecutive compartments.

The ORFs were amplified using two consecutive rounds of PCR amplification from the genomic DNA of Francisella tularensis Schu4.

To obtain triple point mutation, the following mutagenesis primer pairs were used to perform two consecutive rounds of PCR amplification cycles in the HPV-16 E7 cDNA construct plasmid: Double mutation (V55A – F57A).

Thirty to forty consecutive cycles of linear amplification steps were performed.