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In contrast to excitatory connectivity, NGFCs were the most prominent source of inhibition to L2/3 pyramidal neurons, with a connectivity probability of 0.44 (12 connected out 27 connections tested).
Interestingly, none of the BS neurons we recorded were synaptically connected with L2/3 pyramidal neurons (20 connections tested); however, one BS-BS connection confirmed the GABAergic nature of this connection (Supplementary Fig. 8).
The connectivity probability between identified L2/3 pyramidal neurons and L1 interneurons was calculated as the ratio of the number of synaptic connected neurons to the number of connections tested (Fig. 5).
Surprisingly, none of the NGFCs tested received excitatory input from L2/3 pyramidal neurons (68 connections tested), whereas L2/3 pyramidal neurons had a high probability of connectivity with FS cells (0.35, 7/20 connections tested, significantly different from L2/3 Pyr-NGFC ratio, P < 0.001, Fisher's Exact test, and from L2/3 Pyr-c-AC and L2/3 Pyr-BS ratio, P < 0.05, chi-squared test).
After trimming for 6 8 days, connectivity in deprived cortex had returned to control levels (4.0%, 8/201 connections tested, P = 0.988, χ test) (Fig. 3 D).
In contrast, there was a dramatic increase (>3-fold) in Pyr → Pyr connectivity in deprived cortex after 2 4 days of whisker trimming (12.0%, 16/133 connections tested, P < 0.001, χ test) (Fig. 3 D).
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The connection tested was computationally modelled using a finite element computational package, and a good agreement with results of the test was showed.
The experimental details of DWA connection tested previously (Raman 2005) are discussed below briefly.
Malpositioning of the abutment was possible in all systems with a conical implant abutment connection tested.
2) J Integr Neurosci: Given the importance of fronto-parietal connections, we tested whether connection asymmetry affected resistance to distraction.
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