In contrast to excitatory connectivity, NGFCs were the most prominent source of inhibition to L2/3 pyramidal neurons, with a connectivity probability of 0.44 (12 connected out 27 connections tested).
Interestingly, none of the BS neurons we recorded were synaptically connected with L2/3 pyramidal neurons (20 connections tested); however, one BS-BS connection confirmed the GABAergic nature of this connection (Supplementary Fig. 8).
The connectivity probability between identified L2/3 pyramidal neurons and L1 interneurons was calculated as the ratio of the number of synaptic connected neurons to the number of connections tested (Fig. 5).
Surprisingly, none of the NGFCs tested received excitatory input from L2/3 pyramidal neurons (68 connections tested), whereas L2/3 pyramidal neurons had a high probability of connectivity with FS cells (0.35, 7/20 connections tested, significantly different from L2/3 Pyr-NGFC ratio, P < 0.001, Fisher's Exact test, and from L2/3 Pyr-c-AC and L2/3 Pyr-BS ratio, P < 0.05, chi-squared test).
After trimming for 6 8 days, connectivity in deprived cortex had returned to control levels (4.0%, 8/201 connections tested, P = 0.988, χ test) (Fig. 3 D).
In contrast, there was a dramatic increase (>3-fold) in Pyr → Pyr connectivity in deprived cortex after 2 4 days of whisker trimming (12.0%, 16/133 connections tested, P < 0.001, χ test) (Fig. 3 D).
This paper presents test results of two connections tested under cyclic loading.
This study reports a series of 15 experiments on cold-formed steel Z-sections purlins with sleeved bolted connections tested in bending.
