The REE was measured with a calorimeter (Deltatrac™ MBM-100 Metabolic Monitor, Datex-Engstrom Division, Instrumentation Corp. Helsinki, Finland) connected to the ventilator in mechanically ventilated patients.
At time 0, animals were anesthetized, tracheostomized, and connected to the ventilator.
Under isoflurane anesthesia, male adult C57Bl/6 mice were orally intubated and were connected to the ventilator.
Tracheal pressure was measured through an air filled catheter introduced down the endotracheal tube, positioned 2 cm distal to the tube tip, and connected to the ventilator, to obtain alveolar pressure [4].
a Tracheal tube, (b) connection between external cuff and the three-way stopcock, (c) ventilator, (d) three-way stopcock, (e) PressureEasy® device or manometer, (f) pressure transducers connected to the ventilator and to tracheal cuff, and (g) laptop.
Both McMath and Munoz remain on life support, but McMath's parents want to keep their daughter connected to the ventilator, while the Munoz family wants the pregnant woman disconnected from any life-sustaining interventions.
The larger tube is then connected to the ventilator.
The mask is connected to the ventilator, in the same way as an endotracheal tube.
The cuff of the endotracheal tube is inflated and the tube is connected to the ventilator.
As the patient is still connected to the ventilator, mechanics can easily be measured.
