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The vacuum source is connected to a regulator valve with a manometer.
The liquid media was bubbled with medical grade mixture of 99% O2, 1% CO2 flowing from a compressed gas cylinder connected to a regulator, at a 3 L/min rate.
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The thermal regime was controlled by sensors connected to a temperature regulator and recorded using data loggers.
A 9V battery is connected to a 5V regulator (LM340) to power the microcontroller, microphones, amplifiers, and RMS-to-DC converters.
Similarly, we could have had another battery connected to a voltage regulator that could have provided the 5V needed by the microcontroller and the LCD controller.
Bacteria survive under various conditions by sensing stimuli triggering specific adaptive physiological responses, which are often based on membrane-integrated sensors connected to a cytoplasmic regulator.
The computer utilized the oxygen sensor input and controlled two valves via NPN transistors, one connected to an oxygen tank and regulator and one connected to a nitrogen tank and regulator, to maintain specific oxygen concentrations in the box.
Sterile filters from doses (with needle still attached) were connected to a nitrogen supply via a regulator.
The glove circuit is powered by a 9V battery connected to a Freescale battery clip/5V regulator, which was sampled from class.
The source gas (O2 at 345 kPa) was connected to a custom-made pressure regulator and a proportional solenoid valve (SMC 315; SMC Co., Tokyo, Japan).
The oxygen electrode is connected to an oxygen regulator (5) and the regulator is in turn connected to a solenoid valve that regulates the outlet of gas into the aquarium (6).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com