Our spectrum analyzer was placed in a protected room and connected to a high-performance wideband (300 to 3,000 MHz) omnidirectional discone antenna (specifically the Sirio SD 3000N model; Sirio Antenne, Volta Mantovana, Verona, Italy) using a 10-m low-loss RG213-U coaxial cable (see snapshot in Figure 2).
The dialysis tube was directly connected to a high-performance liquid chromatography apparatus (Eicom, Kyoto, Japan) for online analysis of NE, DA, and 5-HT.
For electrospray ionisation mass spectrometry, each of the samples (2 μL) was injected onto a reverse-phase column using a chilled (9°C) autosampler (Eksigent; Dublin, CA, USA) connected to a high-performance liquid chromatography system run at 0.12 μL/minute before a passive split that resulted in about a 400 nL/minute post-split flow (Aligent; Santa Clara, CA, USA).
Hyaluronidase-treated samples (200 μl) were applied to an analytical Superose 6 column connected to a fast-performance liquid chromatography system (former Amersham Biosciences, now GE Healthcare, Uppsale, Sweden) using TBS (pH 7.4) containing 10 mM EDTA and 0.05% emulphogen as eluent at a flow rate of 24 ml/hr.
One option, Krewell says, is to have the notebook run at higher levels of performance when connected to a desktop docking station.
The Mono-Q 5/50 GL Tricorn column (maximum tolerable pressure of 50 Bar) was connected to a modified Agilent 1100 Series high performance liquid chromatography (HPLC) unit.
Sample extracts and calibration solutions (7.5 µl) were injected into a Waters Acquity™ Ultra Performance LC system connected to a Micromass Quattro Ultima triple quadruple mass spectrometer.
Remember, size is not everything; the important thing is that the performance needs to be connected to a truth.
After centrifugation, 0.1 ml of the supernatant was added to 0.1 ml 0.02 M ammonium acetate (pH 4.0), and 0.1 ml of the mixture was applied to a 4.6 × 250 mm Symmetry C18 column connected to an Alliance high-performance liquid chromatography system (Waters, Etten-Leur, The Netherlands).
× 25 cm LC-Si HPLC (high-performance liquid chromatography) column (Sigma) connected to a Shimadzu LC Prominence UFLC solvent delivery system.
