An Agilent nanoflow-1200 series HPLC system was connected to a linear ion trap mass spectrometer (LTQ, Thermo Electron, San Jose, CA, USA).
Peptides were analyzed by nanoLC MS/MS using the Agilent 1100 nanoflow system (Agilent Technologies) connected to a linear hybrid ion trap-orbitrap mass spectrometer (LTQ-Orbitrap XL, Thermo Fisher Scientific) equipped with a nanoelectrospray ion source.
All experiments were performed on a Dionex Ultimate 3000 nano-LC system (Sunnyvale CA, USA) connected to a linear quadrupole ion trap – Orbitrap (LTQ-Orbitrap) mass spectrometer (ThermoElectron, Bremen, Germany) equipped with a nanoelectrospray ion source (Proxeon Byosystems, Odense, Denmark).
The HPLC system comprised of a Dionex (Sunnyvale, CA, USA) 3000 Ultimate series LC connected to a linear ion Trap LTQ-Orbitrap (Thermo Fisher Scientific, USA) mass spectrometer, equipped with an electrospray ion source.
The sensor consists of an array of independent piezoelectric patches connected to a linear combiner.
The nano-HPLC was connected to a linear quadrupole ion trap-Orbitrap (LTQ Orbitrap XL) mass spectrometer (Thermo Fisher).
The linear encoder, connected to a computer measuring system, measured the time and length of the heel displacement of the heel rise.
A real-time B-mode ultrasound (Aloca SSD-210 DXII, Tokyo, Japan) with a 5.0-MHz rectal linear array transducer, connected to a video-recording system was used.
Peptides were analyzed by nanoLC-MS/MS using the Agilent 1100 nanoflow system (Agilent, Palo Alto, CA) connected to a hybrid linear ion trap-orbitrap mass spectrometer (LTQ-Orbitrap XL; Thermo Fisher Scientific, Bremen, Germany) equipped with a nanoelectrospray ion source.
