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To understand these differences, we consider the fact that the estimated internal strain is different for each connect position.
Thus, on neck linker docking, amino acids from the root of the neck linker to the connect position function as an effective lever arm, and the detached head is biased forward a distance ranging from the root to the connect position (hereafter, the distance between the root and connect position is referred to as 'bias length').
Thus, both the connect position and the distance between the two heads can be fully controlled, so one can evaluate the head head coordination mechanism precisely.
In this study, we used the power of DNA-kinesin to evaluate the effects of the connect position and the distance between connecting points.
To do this, we evaluated the effects of both by changing both the connect position and the distance between the heads using constructs with full-length neck linkers.
In this study, however, we presented a novel approach to explore the contribution of the connect position and of the distance between the heads.
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Positions of each partition complex detected in consecutive frames were joined with solid lines while dotted lines were used to connect positions interrupted by frame(s) with failed localization.
To evaluate the other position, we took advantage of DNA-kinesin, which allowed us to make heterodimers with various connect positions (23, 43, 101, 215, 324), whereas the other head was constantly connected at position 337 for full bias in the power stroke.
where, kB is the Boltzmann constant, T the temperature, lp the persistence length, D the distance between two connect positions, L the contour length, and x=D−LDNA where LDNA is length of DNA (end-to-end distance).
Thus, we observed connect positions 333, 328 and 324, in which fluorescent dye labeling had no effect on ATPase or dimer activity (Rice et al, 1999; Tomishige et al, 2006; Mori et al, 2007).
Figure 5② shows the connected status of the blockchain to the double-linked list, and the connected position is indicated by a red box.
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