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The dual-modality molecular probe design also possesses three amino groups for potential conjugation with multiple copies of targeting molecules, thereby providing multivalent effect to the construct for better specific binding to the biological target of interest.
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Exogenous Sp1 pulled-down by FLAG displayed a smear-like SUMO3 conjugation pattern with multiple bands mostly located at molecular weights higher than 130 kDa, confirming that the SUMO3 conjugates of Sp1 were one or multiple polySUMO chain(s) (Fig. 3A).
Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis.
Here is a nice example that illustrates the preceding point: it is from the paper describing the discovery of ubiquitin conjugation as the mark for protein degradation (Hershko A. et al., Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis, PNAS, 1980, April 77 (4): 1783-6).
Here is a nice example that illustrates the preceding point: it is from the paper describing the discovery of ubiquitin conjugation as the mark for protein degradation (Hershko A. et al., Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis, PNAS, 1980, April 77 (Muotri et al, 2005): 1783-6).
They are from two papers on ubiquitin that form the core of the discoveries for which the authors shared the Nobel Prize: 1) Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis.
Unfortunately, for vectors with multiple conjugation sites, the quenching interactions between the dyes present on the same vector can influence the brightness of the tracer.
Conjugation of polymer scaffolds with multiple copies of targeting ligands, drugs, and dyes has become a popular approach for achieving the aim of theranostics: materials useful for both diagnosis and treatment of disease.
This result suggests that extension on the conjugation system of an aromatic compound with multiple electron withdrawing groups can tune the molecule into an air stable ambipolar organic semiconductor.
The degradation of proteins via the protein ubiquitination pathway begins with the conjugation of multiple ubiquitin (Ub) moieties to the target protein followed by the degradation of the polyubiquitinated protein by the 26S proteasome complex.
Apart from the possibility of post-assembly functionalization of PA nanofibers, the NCL conjugation strategy allows simultaneous covalent functionalization of the PA nanofiber with multiple different bioactive ligands.
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