Sentence examples for conjugation using a from inspiring English sources

Their efficient conjugation using a Diels-Alder cycloaddition reaction in aqueous and catalyst-free conditions was also confirmed using N-methylmaleimide as model dienophile.

Following in situ hybridisation on wax sections, mini-Ruby was detected by avidin-biotin-horseradish peroxidise conjugation using a Vectastain Elite ABC Kit (Vector Laboratories), followed by visualisation by using diaminobenzidine-hydrogen peroxide.

For some experiments polytene chromosomes were spread onto microscopic slides: whole cells were gently separated ~48 hours post conjugation using a Heraeus Megafuge at 1000 rpm, 4°C, 7 min. Cells were resuspended in methanol:acetic acid (3:1) and incubated overnight.

Identification and characterization of the location of blaCTX-M-15 was carried out by conjugation, using a nalidixic acid resistant derivative of Escherichia coli HB101 as recipient, PFGE of total DNA of donor and transconjugant digested with S1-nuclease, Southern hybridization with specific probes, and PCR-based replicon typing, as described previously (14 ).

A second method of site-specific drug conjugation uses a microbial transglutaminase enzyme in order to conjugate an amine-containing cytotoxic drug to a mAb modified to have a specific number of glutamine side chains [ 146, 147].

In 1946, Joshua Lederberg and Edward Tatum first described the phenomenon known as bacterial conjugation using E. coli as a model bacterium, and it remains the primary model to study conjugation.

Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester, providing a unique handle for site-specific conjugation using native chemical ligation (NCL).

Recently, Buchwald and co-workers developed a rapid, highly selective cysteine conjugation using aryl palladium complexes (Vinogradova et al., 2015) (Fig. 4C).

Carbodiimide chemistry was applied for conjugation, using N- 3-dimethylaminopropyl -N′-ethylcarbodiimide hydrochloride (EDC) as a zero-leN- 3-dimethylaminopropyl -N′-ethylcarbodiimide

We expect the immunoreactivity benefits of this approach to be more evident on immunoglobulins that are known to lose a significant amount of activity upon conjugation using standard conjugation chemistries.

In this research, a linear DNA probe was immobilized onto nanoparticles through streptavidin conjugation using covalent bonds.