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These findings highlight the significant importance of the multiple σ-π conjugation strategy in designing cost-effective and high-performance σ-π conjugated semiconductors for advanced optoelectronic applications.
Application of this conjugation strategy in selective, label-free nucleic-acid biosensing is also successfully accomplished using a model 16mer PNA probe.
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There have been two strategies developed in the recent literature for quantitative control of the targeting effects for drug delivery by ligand-conjugated nanoparticles of biodegradable copolymer blend such as PLGA/PLGA-PEG, i.e. the pre-conjugation strategy and the post-conjugation strategy, in which the ligand conjugation was made before and after the nanoparticle formulation respectively.
Most successful conjugation strategy was observed in the case of PEI conjugates among which most efficient vector was PEI GO conjugate bearing glycine linker.
The chimeric antibody BR96-doxorubicin conjugate (BR96-DOX) was developed with the hydrazone conjugation strategy.
Generally, the choice of conjugation strategy depends on the presence of intrinsic functional groups in the proteins and haptens.
The carbodiimide reaction is a popular conjugation strategy for introducing haptens on carriers because surface lysines are ubiquitous in proteins.
Nowadays the most widely used method in preclinical Zr-based immuno-PET uses the conjugation strategy with N-succinimidyl-DFO, which addresses ε-amino groups of lysine side chains [ 22, 25– 30].
Studies of pharmacokinetics, biodistribution, and cell cytotoxicity revealed that, employing the polymeric conjugation strategy, the remarkably improved circulatory retention time and bioavailability, as well as reduced peripheral toxicity were obtained in comprising with GA and its Cremophor EL formulation.
This intracellular cleavable conjugation strategy represents a promising approach to improve cancer immunotherapy of soluble vaccines.
The conjugation strategy relies on the direct attachment of FLT3 drugs onto the surface of GNP followed by their subsequent encapsulation in Pluronic as schematized in Fig. 1.
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