We firstly introduced by conjugation a set of 17 conjugative ESBL plasmids (CTX-M-1, -2, -9 and TEM-52) of IncHI2 and IncI1 groups in the SGI1-carrying S. Agona strain 959SA97 previously described to conjugally transfer SGI1 in the presence of the conjugative IncA/C helper plasmid pR55 (Table 1 and data not shown) [15].
Free quantum dots without peptide conjugation were set as a negative control.
Briefly, bi-parental conjugation was set up between the donor E. coli S17-1 strain [55] harboring the pGL10 derivatives (pGC, pGB, pGD, pGCBD, pGECBD, pGECB, and others) and the recipient strains (LC, LB, LD and LCBD).
These examples, as well as numerous genes annotated as "hypothetical" found to be differentially represented among the conjugation EST set, are promising candidates for experimental analysis, using the tools of Tetrahymena reverse genetics [ 1, 89].
The inside cylinder and conjugation assembly were set at an incline of 1 in 8 to clear the front pony truck, leaving space for a third set of independent Walschaerts valve gear between the frames should Holcroft's conjugation mechanism prove unreliable in service.
To set up conjugation, cells of different mating types were mixed.
Although the annotation process suggests that each of the plasmid classes seems to possess a different set of conjugation genes, the fact that all 11 plasmids are related, sharing large blocks of DNA sequence tends to suggest that the conjugation systems themselves will also be functionally closely related.
Intriguingly, although ubiquitylation was directed at K164 of PCNA, the modification did not involve the set of conjugation factors required for damage-induced ubiquitylation, but instead the Cullin 4-based ubiquitin ligase CRL4, which is known for assembly of K48-linked ubiquitin chains (Lo et al. 2012).
The strain IOEB_C52 contains a contig with genes that are typical of conjugative plasmids: a complete set of the Trs proteins, conjugation proteins, integrases, and transcriptional regulators.
In Sumner et al. [ 27], we gave some additional insight into the interpretation of applying the Hadamard conjugation in a network setting.
