Sentence examples for conjugation procedures from inspiring English sources

The authors also noted that photostability of the QD conjugates was an issue during preparation, and QD conjugation procedures were performed under little or no light to minimize QD photolysis.

Because both recognition and readout capabilities are incorporated into a single DNA molecule, tedious conjugation procedures required for protein DNA hybrids can be omitted.

However, commercial HRP preparations are typically isolated from horseradish roots as varying mixtures of HRP isoenzymes with different biochemical properties, and chemical conjugation procedures lead to heterogeneous HRP-SpG preparations, partially including inactivated enzyme.

Here we review methods for non-covalent and covalent chemical modifications of actin filaments with focus on critical advantages and challenges of different methods as well as critical steps in the conjugation procedures.

Despite the short incubation time, the conjugation reaction is sufficient to achieve conjugates with performance characteristics identical to, or better than, those prepared with laborious multistep conjugation procedures.

Importantly, all the conjugation procedures were implemented in physiological reaction environment (aqueous solution, neutral pH), in order to develop a quite general platform to be used for a wide range of applications involving biomolecules such as solid-state biorecognition of target analytes (DNA hybridization, protein/antibody interactions).

The following conjugation procedure has been used to conjugate COOH group-containing photosensitisers (verteporfin and SnCe6) to fVII protein using crosslinker EDC (N'-3-dimethylaminopropyl N-ethylcarbodiimide hydrochloride; Sigma), as described in detail by Hu et al (2010a, 2010b).

Intermediate 4 (4.2 mg, 7 μmol) was conjugated with DOTA-NHS ester (6.4 mg, 8 μmol) using a conjugation procedure similar to that for 3. Compound 5 was obtained after HPLC purification using Method-2 (5.5 mg, 5.5 μmol, yield = 79%).

Parameters included choice of antibody construct, antibody density, antibody conjugation procedure, and choice of liposome construct.

In addition, we simplified the conjugation procedure without heat-shock and pre-germination treatments but with high conjugation efficiency, suggesting it is inherently capable of accepting heterologous DNA.

To date, the effort has been mostly limited on coating iron oxide particles with a thin layer of Au, where the Au shell not only provides convenient anchorage sites for functionalization of biomolecules through the well-established Au-thiol conjugation procedure but also protects SPIONs from dissolution and aggregation.