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The CP self-assembles into ~ 40 nm diameter nanoparticles upon conjugation of multiple copies of doxorubicin (Dox), and displays a nanoparticle-to-aggregate phase transition between 39 and 42 °C in media, a temperature range suitable for mild hyperthermia of solid tumors.
The CP self-assembles into ~40 nm diameter nanoparticles upon conjugation of multiple copies of doxorubicin (Dox), and displays a nanoparticle-to-aggregate phase transition between 39 and 42 °C in media, a temperature range suitable for mild hyperthermia of solid tumors.
The degradation of proteins via the protein ubiquitination pathway begins with the conjugation of multiple ubiquitin (Ub) moieties to the target protein followed by the degradation of the polyubiquitinated protein by the 26S proteasome complex.
Mier et al. studied the stochastic conjugation of multiple dyes to PAMAM dendrimer including fluorescein, rhodamine, coumarin, and dansyl.
We hypothesized that the conjugation of multiple peptides onto a single scaffold would allow the antigens to be taken up and presented at a higher density to overcome the activation threshold of mouse CD4 T cells.
Our group recently showed that conjugation of multiple HIV-TAT epitopes to PEI2K PEG5K 10 copolymers greatly improved ESO delivery, using a similar dosing and intramuscular injection regimen as reported here, resulting in up to 30% dystrophin expression (Sirsi et al., manuscript submitted).
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Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis.
As expected, no BPA conjugation was evident in cells lacking TG2 expression (Vec) or harboring catalytically inactive mutant protein TG2-C277SS), whereas TG2-WT and TG2-R580A-transfected TG2-R580A-transfected TG2-R580A-transfected to multiple cellslar proteinshowed
Here is a nice example that illustrates the preceding point: it is from the paper describing the discovery of ubiquitin conjugation as the mark for protein degradation (Hershko A. et al., Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis, PNAS, 1980, April 77 (4): 1783-6).
Here is a nice example that illustrates the preceding point: it is from the paper describing the discovery of ubiquitin conjugation as the mark for protein degradation (Hershko A. et al., Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis, PNAS, 1980, April 77 (Muotri et al, 2005): 1783-6).
They are from two papers on ubiquitin that form the core of the discoveries for which the authors shared the Nobel Prize: 1) Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis.
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