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In addition, several genes associated to plasmid conjugation (mobC, traC, traD, traL, traN, traX) are present in the pEIB202 sequence, demonstrating the genetic basis for its capability to transfer between bacteria in the laboratory system with a conjugation frequency of 1.6×10−6 (data not shown).
Let βl and βh be the conjugation frequency of the low copy number and high copy number plasmids (plasmid lc and hc respectively).
Compared to the previously reported conjugation frequency of 9.0·10−2 transconjugants per donor in the plasmid pOLA52 [25], the observed transfer frequencies of this study suggest that the conjugation apparatus of pX1.0 functions similar to that of pOLA52.
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Similar conjugation frequencies of plasmid pCLL from the nonproteolytic strain Eklund 17B were observed when either strain LNT01 or Hall A-hyper/Tn916 was used as recipient (Table 2).
To verify functionality of the combined 15,827 bp region containing the tra- and mob modules, conjugation frequencies of pX1.0 from E. coli GeneHogs® donors into different members of Enterobacteriaceae family, were determined.
Intraspecies conjugal transfer of plasmids in C. perfringens has been reported to be a highly efficient process with conjugation frequencies of 10−1 to 10−2 transconjugants per donor [25], [26], [27].
Variations in the conjugation frequencies of the more distant genomic islands could be attributed to the slight differences in the gene content of their T4SS modules; however, this explanation would not suffice for the closely related islands with the same set of 24 GI T4SS genes like ICE Hin1056 and ICE HpaT3T1 (Fig. 3).
Conjugation frequencies of pBF3 into S. coelicolor and S. lividans were reminiscent of those obtained with ϕBT1 and ϕC31 integration plasmids; the numbers of hygromycin resistant exconjugants for S. coelicolor J1929, S. coelicolor M512, and S. lividans TK24 were greater than 1 × 10 (Table 2), and the integrations were stable after two rounds of sporulation without selection (see below).
With E. coli S17-1 (DNA methylation proficient) or E. coli ET12567/pUZ8002 (DNA methylation deficient) as donors and S. amphibiosporus spores as recipients, integrative plasmids such as pSET152 can be introduced into S. amphibiosporus with conjugation frequencies of 10 6 or 10 7, respectively (Table S1 of the Supporting Information).
The conjugation frequencies for pBotCDC-A3-Erm and of pCLJ-Erm increased markedly when Hall A-hyper/Tn916 was used as the recipient.
If one considers the high conjugation frequency and stability of the IncA/C plasmid containing NDM-1 in Salmonella spp., one would conclude that it might increase spread of bacterial drug resistance.
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