Through conjugation experiments, none of the 24 conjugative plasmids tested of the IncFI, FII, HI2, I1, L/M, N, P incompatibility groups were able to mobilize SGI1 at a detectable level (transfer frequency <10−9).
Conjugation experiments revealed the presence of a large conjugative plasmid (pLM07/20) with an exclusive FrepB replicon type (IncF/FIB).
Conjugation experiments and plasmid analysis demonstrated the prevalence of conjugative resistance plasmids TP17 and TP19 among E. coli isolates.
Furthermore, conjugation experiments were performed obtaining 30 transconjugants when selecting for antimicrobial resistance.
We then conducted conjugation experiments using 4-nm QDs to verify the existence of Au on these tips.
Using the model and a series of chemical conjugation experiments, we identified and probed the addressability of cysteine side chains.
In conjugation experiments performed on solid medium, pSD107 was successfully transferred to a nalidixic acid resistant E. coli DH5α, mobilizing pSD4.0 and, more infrequently, also pSD4.6.
Conjugation experiments showed that it is transferable.
The potential for the transfer of resistance determinants was investigated by conjugation experiments.
Conjugation experiments were performed overnight at 37°C with a donor/recipient ratio of 0.2.
Conjugation experiments were conducted using different antibiotics in combination with sodium azide for selection of transconjugants.
