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Crucial for these studies is the similar conjugation efficiency of the fluorophore to the two VHHs.
Conjugation efficiency of IRDye800CW to 89Zr-mAbs was approximately 50%; on an average, 0.5 to 5 eq IRDye800CW was conjugated.
HPLC analysis confirmed that there was no difference in the conjugation efficiency of IRDye800CW to cetuximab/bevacizumab or 89Zr-cetuximab/bevacizumab.
For the imaging probe, the conjugation efficiency of IR to cetuximab was approximately 70%, resulting in 1.4 groups of IR per molecule cetuximab.
The conjugation efficiency of folic acid to CNPs as determined by UV Visible spectrophotometer revealed that ∼0.33 mg of FA was conjugated per milligram of CNPs.
Since CC49 and QDs used for conjugation were 8 1 (mol/mol), the real conjugation efficiency of CC49 and QDs was dramatically high.
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Subsequent coupling of IRDye800CW to the radioactive conjugate gave conjugation efficiencies of about 50%, resulting in IRDye800CW-to-mAb molar ratios of 0.5 1 to 5 1, as assessed by HPLC analysis.
The conjugation efficiencies of different sizes of GSBP-PEG, as well as different end-functionalized 5K PEGs, are summarized in Table 1.
In contrast to Plasmodium ATG8, a significant population of GFP-TgATG8 is detected in the cytosol, but this may be due to overexpression and/or tagging of GFP.,, In general, the PE-conjugation efficiency of the Atg8 family proteins is reduced when they are tagged with large molecules such as GFP.
Either NHS or NHSS may be used to improve the coupling efficiency of EDC-mediated protein-carboxylic acid conjugations.
This attractive TX ability stems from combination of its (i) high conjugation efficiency in each of the two planar moieties, (ii) intrinsic 3D structure on account of the presence of the central node, and (iii) intrinsic regioselectivity in electropolymerisation on account of the positions of the two available free α-thiophene sites.
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