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The efficiency of conjugation does not bear a saturating relationship with realized fitness and therefore both low and high copy number plasmids should evolve conjugation machinery with high efficiency.
However, because of the nature of the cyclic system, the conjugation does not extend from one thiol group to the other.
Although there appears to be common pathways involving ubiquitin and ISG15, conjugation does not increase the degradation of ISGylated proteins [ 45, 46].
First, the activity of the protein polymer bioconjugates was compared with that of the wild type nonconjugated sfGFP, in order to confirm that polymer conjugation does not affect the inherent fluorescence of the protein.
However, DM1 conjugation does not alter the internalization of anti-CD19 antibody (data not shown), whereas ricin conjugation to another anti-CD19 antibody increases its internalization, possibly via toxin dimerization and CD19 cross-linking (Goulet et al, 1997).
The fact that we did not find mutants severely affected in these early stages suggests that conjugation does not require an active involvement of the recipient in the transport process.
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The structures of the conjugates have not been established conclusively but the conjugation did not appear to involve the epoxide group (Gardiner et al. 2010).
Ligand conjugation did not significantly change the physicochemical characteristics of liposomes.
Furthermore, the fatty acid conjugation did not cause a significant reduction in the fluorescence of sfGFP.
Retinal conjugation did not dramatically alter the surface charge (−24 ± 2.0 mV).
The authors concluded that paclitaxol was released in the cells, and that polymer conjugation did not affect the activity of paclitaxol.
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