MALDI-TOF is the most common method used to determine the protein−carbohydrate conjugation efficiency based on the mass shift before and after conjugation.
The two T4SSs in HAMBI 1141 belong to the quorum sensing (QS -regulated conjugation system (type I) and a type IV conjugation system, based on homology to systems assigned to these types defined by both structural and phylogenetic analyses by Ding and co-workers [ 5].
The second enzyme layer was deposited using the protein conjugation method based on the high affinity "avidin biotin" interactions.
The drugs were loaded onto the surface of nanoparticles using a conjugation strategy based on hydrophobic-hydrophobic interactions with the Pluronic co-polymer used as nanoparticle surface coating.
Park et al. also demonstrated a conjugation method based on streptavidin/biotin reagents for strong attachment of the bacteria and microstructures by a high-affinity interaction.
The drugs were loaded onto nanoparticle surface using a conjugation strategy based on hydrophobic-hydrophobic interactions with the Pluronic co-polymer used as nanoparticle surface coating.
The final method of drug conjugation is based on the use of non-natural amino acids, such as selenocysteine or acetylphenylalanine [ 151, 152].
Two of the three high-level mupirocin-resistant isolates (A15 – South Africa and 35 IBA – Nigeria) were selected for curing and conjugation experiments based on their antibiotic resistance profile.
