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The GSH and NAC conjugates were shown to result from conjugation at the C10 position of B[ a]P-7,8-dione, Figure 8.
All three unmodified ubiquitin NoxaBH3 conjugates showed >5-fold higher activity than the parent NoxaBH3 peptide, with ubiquitin conjugation at the C-terminus of NoxaBH3 peptide being preferred (Table 2).
In the recent years, protein PEGylation has become an established and highly refined technology by moving forward from initial simple random coupling approaches based on conjugation at the level of lysine ε-amino group.
These results are analogous to protein ubiquitination in endoplasmic reticulum associated degradation (ERAD), where both soluble and membrane inserted ER proteins undergo retro-translocation before ubiquitin conjugation at the cytosolic face of the ER [13], [14].
Ciliates can engage in monogamous sexual-like conjugation at the end of their lifecycles.
For quercetin (256, 372) only the product P1 (256, 372) did not show a hypsochromic shift suggesting conjugation at the 7-hydroxyl group.
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Poor matching of the HOMO energies of the two components, together with cross-conjugation at the alkene bridge appear to limit charge carrier mobilities.
Series of 1,8-diazabenzopyrenes and 1,7-diazaperylenes with thienyl or 4- phenylamino phenyl groups were designed and synthesized by a one-pot process from the functionalized terminal alkynes and either 1,4- phenylamino phenylaquinones in order to investigroupshe effect of extending π-conjugation at the 2,7- and 2,8-positions on the optical and electrochemical properties.
For chemical conjugation of SHe to keyhole limpet hemocyanin (KLH), we used a SHe peptide variant (CGGGS-NKLSEYNVFHNKTFELPRARVNT) in which the cysteine at position 4 in the natural SHe amino acid sequence was substituted by a serine to prevent conjugation at this position.
Following production of protein polymer bioconjugates, we then carried out a series of characterization experiments to assess the impact of conjugation at different sites on the protein surface on the macromolecule properties of the bioconjugates.
Briefly, assays were performed by recording the first 2 min of GS-CDNB conjugation at 25 °C in the reaction mixture consisting of 100 µL of 50 mM GSH (Sigma-Aldrich, Taufkirchen, Germany), 40 µL of 50 mM 1-chloro-2,4-dinitrobenzene (CDNB) (Sigma-Aldrich, Taufkirchen, Germany) dissolved in ethanol, 10 µL of enzyme extract and 0.1 M potassium phosphate buffer, pH 6.5, in a total volume of 2 mL [24].
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