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By conjugation, 1 plasmid of ≈50 kbp was successfully transferred into an E. coli J53 recipient.
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UBE1L and UbcH8 have been identified as the specific E1 and E2 enzymes for ISG15 conjugation respectively [9], [13], [14].
Related mutants were used in HeLa cells to identify 14 SUMO-1 conjugation sites, 194) in HEK293 cells to identify 17 SUMO-3 conjugation sites, 166) and in A. thaliana to detect 17 SUMO-1 conjugation sites.
In addition, all members of the Atg8 conjugation system (Atg7, Atg3, and Atg4) were detected.
UbcH8 has been identified as a major Ub-Conjugating enzyme effecting ISG15 conjugation [12], [13].
The substitution at the 2,6-position is expected to provide high degree of planarity and effective conjugation [13, 15].
In the highly aggregated configuration, the polymer chains may align themselves and increase the conjugation length[18].
We analyzed also the effect of Ufl1 knockdown on apoptosis, to be able to investigate the importance of the conjugation between UFM1 and UFBP1, since a reduced UFL1 expression results in a significant reduction of UFM1-UFBP1 conjugation [1].
Mutation of these residues greatly reduced Nedd8 adenylation and consequently conjugation [36].
The mutator plasmid (pLRI-7) was introduced in BAB-parental by conjugation [78].
Recently, we and others showed that SnoN is sumoylated and that Lysines 50 and 383 on SnoN are major sites for SUMO conjugation [38], [50].
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