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Fig. 1 PAGE of conjugated sample.
Both bands in the SUNBRIGHT® ME-050MA conjugated sample were found in the materials co-immunoprecipitated with wild-type hFasRECD-T-Fc protein.
On the other hand, SUNBRIGHT® ME-050MA conjugated sample eluted markedly early (22.26 min) and the peak retention time was even significantly earlier than the 158 kDa size-marker (25.04 min).
In this process, if the lifetime of the donor is extremely short relative to the lifetime of the acceptor, no appreciable energy transfer occurs Fig. 9 Luminescence lifetimes at 600 nm following excitation at 285 nm for CdTe, a CdTe-FA conjugated sample, and a mixture of CdTe and folic acid.
The peak retention time of the N-Ethylmaleimide conjugated sample (28.78 min) was between the size-markers of 44 kDa (30.22 min) and 158 kDa (25.04 min), and was nearly the same as observed for tag-free hFasLECD (29.55 min) under the identical elution condition using the same chromatography column.
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Conjugate, sample, and absorbent pads as well as the nitrocellulose (NC) membrane and polyvinyl chloride (PVC) plate were purchased from JieYi Biotech Co., Ltd.
The second addend has the same distribution as well, since under the condition (11), vectors ( {boldsymbol{Pi}}_Mcdot {mathbf{y}}_i^{sim}kern0.5em left(iin 1,Kright) ) of the "reverse" and complex conjugate sample Π M ⋅ Y ~ possess the same properties as the initial vectors, y i.
AUC of each HA-incubated conjugate chromatogram was normalized to percent AUC of conjugate sample in the absence of HA used as control.
After incubation with rat monoclonal anti-HA (Roche) and a rabbit anti P. berghei MSP1 polyclonal serum (de Koning-Ward et al, 2003) followed by Alexa Fluor 568 and 647 conjugated antibodies, samples were stained with DAPI and mounted in ProLong® Antifade kit (Molecular probes).
The purity of the conjugate, FITC-labeled Vg, was confirmed by native-PAGE and Coomassie blue staining, and its F/P ratio (F/P ratio is defined as the ratio of moles of FITC to moles of protein in the conjugate) was calculated by the equation F/P = (2.77×A495)/[A280-(0.35×A495)] from the absorbance readings of the conjugate samples [43].
An LOI value of 26.6% and a UL-94 V-0 rating can be achieved when the PWPU sample conjugated with 12 wt% EPPD, showing good inherent flame retardancy.
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