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The precursor of LC3 (light chain 3), a homologue of the yeast autophagy marker ATG8 and an interactive protein of the MAP1 (microtubule-associated protein 1) family [ 11– 13], is truncated to form the cytosolic LC3-I and conjugated further with phosphatidylethanolamine to create the isolation-membrane-associated LC3-II with the assistance of ATG5 ATG12 conjugates [ 14, 15].
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The Atg12-Atg5 conjugates further associate with Atg16 to form a multimeric complex, which regulates Atg8 PE conjugation and its localization.
To investigate the effect of MK571 on the glucuronidation and sulfation of individual kaempferol conjugates further, a range of concentrations of aglycone were incubated with MK571 and the cell lysate.
The conjugated antibodies further enhanced uptake and cytotoxic potency of the nanoparticle.
Since previous studies [13], [23] found that this procedure produced successful nanotube-oligonucleotide conjugates, further purification and characterization was not performed.
[Editors' note: further revisions were requested prior to acceptance, as described below.] Thank you for resubmitting your work entitled "Wss1 metalloprotease partners with Cdc48/Doa1 in processing genotoxic SUMO conjugates" for further consideration at eLife.
For SRBC-OVA conjugation, OVA protein (Sigma-Aldrich) was first cross-linked with HEL with glutaraldehyde and further conjugated to SRBC in a same manner as SRBC-HEL2× conjugation.
Hb was further conjugated into the swelling FNGs by Schiff base reaction under mild condition.
The TPGS2k micelles were further conjugated to folic acid (FA) for targeted drug delivery.
The prepared NPs were further conjugated to anti-EGFR antibody using glutaraldehyde-mediated cross-linking described previously.
M-PAEEP-PLLA-NPs were further conjugated with Lf (Lf-M-PAEEP-PLLA-NPs) for glioma tumor targeting.
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