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A biotinylated poly (dT) control for conjugate reaction was applied to each strip to ensure the validity of the test and proper alignment of the strips on the interpretation sheet.
A biotinylated poly dT) control for conjugate reaction is present in each strip to ensure good test performance and a proper alignment of the strips on the interpretation sheet.
After washing the column three times with PBSM buffer (10.1 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl and 1 mM MgCl2, pH 7.4), we loaded the conjugate reaction mixture onto the center of the resin.
Dual binding between tumour cell surface antigen and MTX was demonstrated by the ability of the hybrid antibody to bridge between tumour cells and MTX as MTX-HSA conjugate, reaction here being detected by flow cytofluorimetry.
After hybridization of the PCR product to the probes on the strip under stringent conditions, followed by stringent washing, the hybrids were detected by alkaline phosphatase/streptavidin conjugate; reaction with a substrate (5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium) results in a purple precipitate at the positive probe lines.
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Hayashi, T. & Berthon, G. Catalytic Asymmetric Conjugate Reactions Wiley-VCH Verlag GmbH & Co. KGaA (2010).
It means the conductive zone of anatase type is 0.2 eV higher and therefore is more favorable for driving conjugate reactions involving electrons.
Sepharose CL-4B chromatography of NP conjugate reactions showed that NP eluted between 20 ml and 25 ml and the free IgG between 45 ml and 60 ml (Figure 1, top).
From these results, a new assay method for quantifying lysozyme was established by utilizing the Morgan-Elson reaction based on the generation of product D (2-acetamide-2,3-dideoxy-glucopyranose), which serves as a chromophore, formed from Gal(GlcN 3D by lysozyme through a conjugated reaction involving β-N-acetylhexosaminidase.
Processes involving coupling reactions of isolated alkenes, α,β-unsaturated carbonyl compounds, or isolated alkynes will not be discussed here, including asymmetric Heck and oxidative Heck reactions, conjugate addition reactions, and asymmetric ring-opening reactions of oxabicyclic compounds.
The analysis showed that the tested conjugation reaction yielded fluorescent conjugates of the dye through carbodiimide chemistry.
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