Sentence examples for conjugate buffer from inspiring English sources

After washing as described earlier, HRP-conjugated streptavidin (21130; Pierce) was diluted 1 60,000 in the conjugate buffer and incubated with the plate for 1 hour at room temperature.

For automated evaluation, digital image taking was performed on tissue sections of monkey esophagus first using diamidino phosphatidyl inositol (DAPI) added to the conjugate buffer for image focusing on cell nuclei as reported elsewhere [29, 30].

Standard (range 100 ng/ml-0.14 ng/ml) and athlete serum samples were diluted 1 10 in conjugate buffer (Medicago 25-0142+ 0.1 mg/ml goat IgG from Sigma) and pre-incubated with HRP-coupled anti-CILP-2 antibody (polyclonal affinity-purified goat antibody (Capra Science, Sweden) against the coating CILP-2 peptide) on non-binding plates (U96 PP 0.5 ml, Natural, Thermo Scientific) for 1 h.

A biotinylated polyclonal anti-C3d antibody (A0063; Dako) diluted 1 1,000 in the conjugate buffer of the kit, was added to the wells, and the plates were incubated for 1 hour at room temperature.

After washing twice, serial dilutions of human sera, starting at 1 100, in 100 μl conjugate buffer (1% BSA, 0,05% Tween in PBS) were incubated for 1.5 hours at 37°C.

After four wash steps, alkaline phosphatase coupled goat anti human Ig (IgM + IgA + IgG, Dianova) or goat anti human IgG (Dianova) diluted 1 2000 in 100 μl conjugate buffer was added.

The main reason for these differences is that although the antigen is the same, the solid support materials might be different - and the added variables (conjugate, buffers, incubation time, and so forth) are also different, and these may also contribute to the small differences reported.

The wells were washed twice with wash buffer and then incubated with a simultaneously blocking and conjugating buffer at 30°C for 15 min on a shaking thermomixer.

Following treatments with Lexa or Lexa combined with CHX, cells were washed with conjugating buffer containing 150 µM NaCl, 20 mM HEPES (pH 7.2), 1.5 mM MgCl2, and 10 mM glucose.

The uncojugated ROS was removed from the ROS-protein conjugates by buffer exchange using a Centricon-30 filter.

The stability of the conjugates in buffer and in mouse sera was measured by HPLC at room temperature (RT) over a period of 1 to 120 hrs.