Add approximately 100 g of cyst material in a 250 ml graduated conical shaped container filled with brine.
The conical shape of the container allows the sample to rest stably with a minimum amount of medium surrounding it.
For safety and containment of hazardous infectious materials, the syringe or grid should be placed in a rigid sterile container, e.g., conical 15-mL centrifuge tube or Beem capsule (Beem Co., Bronx, NY), sealed with Parafilm (American National Can Co., Greenwich, CT), and the outside of the tube washed with 0.5% sodium hypochlorite (10% household bleach) before transport.
The experiment was conducted using three 2-L, conical-shaped glass containers placed in an incubator tank to determine the mortality of unfed larvae during their yolk stage.
Best hatching results are achieved in containers with a conical bottom, aerated from the bottom with air-lines (Fig. 4.3.1.).
The centerpiece of this large, highly sophisticated cedar-plank dwelling -which included containers of whale oil, harpoons and conical rain hats made of woven spruce roots -- was a piece of artwork: "the giant, carved-from-wood dorsal fin of a whale, decorated with some 700 otter teeth, the prize in the house of a whaler".
Pass through through a fine sieve (or, if you have one, a chinoise – that's the posh term for those conical sieves cheffy types use), tip into a freezer-proof container and pop on the lid.
Milk samples were self-collected manually into breast pumps with glass containers at the individual hospitals and transferred to 50-mL polypropylene conical tubes (milk tube) that had been thoroughly rinsed with methanol and acetone before use; samples were kept frozen at –20°C.
A 0.5 m×250 m, 250 µm conical plankton net mounted on a fixed metal frame with a removable, weighted cod-end sample container was lowered to a depth of 20 m (near bottom) and pulled up at a steady 1 m·s−1 rate to the surface.
This is enabled since the conical design allows natural convection to dominate in a large volume of PCM at top of the container.
