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Exact(7)
K-mean clustering method was used to generate the ligand perturbation ensemble and similar/redundant conformations (rmsd to the selected conformation is less than 0.5 Å) were omitted.
The results are not unexpected as conformation is less likely than other findings to be influenced by management strategies or age.
In agreement with our hypothesis, Fam20A Q258E, which is able to hydrolyze ATP and therefore will not be locked in an ATP-binding conformation, is less potent than WT Fam20A to activate Fam20C.
In agreement with the potential role of ATP in stabilizing Fam20A conformation, Fam20A Q258E, which is able to hydrolyze ATP and therefore will not be locked in an ATP-binding conformation, is less potent than WT Fam20A to activate Fam20C.
In the NP4(L133V -NO structure (pH 5.6), the G H loop is more disordered than in the wild-type structure and the closed conformation is less well-defined in the electron density map.
The FEL for 2 reveals that the conformations relevant to the reaction coordinate are all energetically accessible, and moreover, that a B2,5 conformation is less stable than the E and H conformations.
Similar(53)
Not surprisingly, finding a conformation with a low RMSD from the reference crystal conformation was less probable for highly flexible ligands (n = 147; 7 ≤ #RotB ≤ 10).
Interestingly, PLMs QRATKM and RRATKM, which do not adopt a canonical helical conformation, are less potent (Ki values = 133 µM and 95 µM, respectively) than the 'helical' PLMs JTH-NB72-39 JTH-NB72-39 JTH-NB72-39 whI1h possess nM rande Ki values (Table S1).
Gaseous ions formed from solutions in which a protein has a native or globular conformation are less highly charged than the corresponding ions formed from solutions in which the protein is unfolded.
The RMSD analysis of the backbone atoms at 300 K showed that values converged at the relatively low level of 5 Å, indicating the protein conformation was less deviated from its initial structure (see Fig. 1).
With respect to cold and non-thermal stress treatments, however, the impact on cellular protein conformations is less direct and not as well understood.
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