The results indicate that, despite making up only a small fraction of the polymer, the terminal groups play a large role in both the conformation and assembly of PNIPAM and its analogs below the critical temperature, and hints at the possibility of new design strategies for thermoresponsive materials.
SAXS data were collected at beamline 12.3.1 of the Advanced Light Source, Lawrence Berkeley National Laboratory, at 12 keV on a MAR165 detector and used to analyze the solution architecture, conformation, and assembly of BARD1 samples.
Hammel, M. et al. Ku and DNA-dependent protein kinase dynamic conformations and assembly regulate DNA binding and the initial non-homologous end joining complex.
X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
Interestingly, the A-subdomain of Mcm2 interacts with Cdc45 (Costa et al., 2011), providing a potential link between A-subdomain conformation and CMG assembly.
Through a combination of the most recent T4 structures with previous structural and biochemical data, many general features of clamp loader structure and function can be placed in the context of detailed structural models for changes in conformation and the assembly of complexes.
Nevertheless, the observed correlation of electrostatic interaction with fibrillation propensity of tau40 might indicate plausible link between hyperphosphorylation at diseased state with tau conformation and self-assembly.
Förster nonradiative energy transfer (FRET) technique has been successfully used to probe biological systems to study the structure, conformation, spatial distribution, and assembly of complex proteins [ 26].
We investigate the assembly, morphology and tunability of the nanostructure of adsorbed rec1-resilin architectures by atomic force microscopy (AFM) and scanning thermal microscopy (SThm) demonstrating that the protein conformation and structure during assembly can be controlled by tuning the physical conditions at the surface.
HMG proteins, including HMGA2, cannot initiate transcription, but instead are involved in dynamic changes in DNA conformation and orchestrating the assembly of multicomponent nucleoprotein complexes that participate in gene replication and transcription.
1, 2, 4 The synthetic versatility of peptoids has also enabled recent studies on controlling polymer chain conformation and nanostructure self-assembly by monomer sequence design.
