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Cells at 80 90 % confluence were used for all of the experiments.
Adherent mammalian cells to 75% confluence were cultured in 6 well plates with coverslips.
Briefly, MDA-MB-231 cells or LM2 cells at 80% 90% confluence were seeded in a 96-well plate.
For internalisation, PC-3 cells at confluence were placed in six-well plates and left to attach overnight.
The cells grown to 80% confluence were used for experiments.
Growing cells (60% confluence) were treated with 7.5 µM of osajin for 24 h.
C2C12 cells grown to 90% confluence were induced to differentiate by serum withdrawal.
Exponentially growing HCT116 p53+/+ cells at 50 80% confluence were used for protein fractionation.
Cultured cells that had reached ∼70% to 80% confluence were used for protein analyses.
Approximately 120,000 cells (about 30% confluence) were plated per well for all transfection experiments.
For each IP experiment, 6 10 cm dishes of HeLa cells at 70% confluence were used.
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