Sentence examples for confluence was obtained from inspiring English sources

As growth continued, adjacent colonies interconnected with each other and a monolayer confluence was obtained after 12 ~ 15 days of incubation.

Media and supplement exchange were performed when 90% confluence was obtained.

During culture, adjacent colonies interconnected with each other, and a monolayer confluence was obtained after 12 15 days of incubation.

Myogenic differentiation of C2C12 cells at ∼80% cell confluence was obtained by substituting the medium with fresh medium supplemented with 2% horse serum (EuroClone, Milan, Italy) to induce expression of mutant SOD1 under the myosin heavy chain promoter.

The measurements were performed on undifferentiated HMSCs after confluence was obtained and on neuroglial-differentiated HMSCs, cultured on Vivosorb discs in order to correlate the HMSCs ability to differentiate and survival capacity in the presence of the Vivosorb membrane).

Cell lines were subconfluent, and each cell line has been optimized in previous titration experiments so that cell type-specific optimal conditions for the seeding rate and confluence were obtained for the real-time measurement in these experiments.

Autologous (n = 7) and allogeneic (n = 5) donor MSCs were grown to 70% confluence and the conditioned medium was obtained.

The mouse macrophage cell line, RAW 264.7, was obtained from American Type Culture Collection ATCCC, Rockville, MD, USA), and cells were maintained in DMEM/F12 containing 10% FBS until confluence to cell monolayer.

HUVECs were grown in 24-well plates at 37°C in complete growth media at 80% confluence; the concentrated conditioned media were obtained from K562 cells transfected with pVAX-sFLT-1 or pVAX-GFP in triplicates.

When MCF-10A cell confluence reached about 80%%, single cells were obtained by 0.25 % typsin/EDTA (Gibco, USA) digestion, stained by MUC1-PE (BD Pharmingen, USA) and ESA-FITC (BD Pharmingen, USA).

After reaching confluence in the primary culture, serial passages were obtained, always applying a 1 3 split ratio.