Sentence examples for confluence used from inspiring English sources

c-Myc expression decreased strongly with increasing cell confluence with highest c-Myc levels around 50% confluence (used for further experiments).

Cells were passaged three times upon reaching 70%to80%0% confluence using Accutase.

The cells were harvested after attainment of 75-80% confluence using trypsin (0.025%) and EDTA (0.52 mM) in phosphate buffered saline (PBS).

A549 cells were infected when they reached 70 80% confluence, using different multiplicities of infection (MOI).

Transfection was carried out at approximately 50% confluence using the Calcium phosphate method following standard procedures.

Cos-7 cells were cultured in DME/5 % fetal calf serum and transfected at 90%% confluence using Lipofectamine 2000 (Invitrogen).

Cells were transfected at 60 80% confluence using 1 µl of FUGENE 6 (Boehringer Mannheim, Germany) and 0.5 µg of DNA/1×105 cells in 1 ml of culture medium [33].

HUVEC were transfected at 70 90% confluence using the Lipofectamine and the PLUS reagent (Invitrogen, Carlsbad, CA, USA) for 1 h at 37°C in DMEM.

Total RNA was isolated from astrocytes cultured in T-75 flasks (P3, 70 80% confluence) using the Qiagen RNeasy Kit protocol for adherent cells.

For transfection experiments, cells were plated on glass coverslips and transfected at 70 90% confluence using Lipofectamine 2000 (Invitrogen) following the manufacturer's instructions.

10 T1/2 fibroblasts and HeLa cells were transfected in 24-well plates at 40% confluence using Lipofectamine 2000 (Invitrogen) and harvested 36 h after.