Sentence examples for confluence upon from inspiring English sources

Cells were plated in 35-mm petri dishes the day before transfection so that they reached 60 80% confluence upon transfection.

Culture medium was subsequently changed every 48 to 72 h until synoviocytes reached confluence, and upon confluence the synoviocytes were passaged using 0.25%% trypsin/1 mM EDTA (InvitrogenTM).

In healthy cultures, our cell line grows to confluence and upon differentiation forms an electrically resistant monolayer supporting directed salt and water transport [ 6].

Conversely, we found that a striking majority of genes whose expression increases upon confluence (day −2 expression lower than day 0 expression) were downregulated at day 0 upon ZEB1 KD.

Upon confluence (rare), HDFs and OSTs were trypsinized and resuspended in the medium at a concentration of HDFs (103 cells in 30 µl medium on 0.5 × 0.5 cm substrates) and OSTs (3 × 104 cells in 30 µl medium on 0.5 × 0.5 cm substrates) which were chosen to give the maximal response without restricting their spread ability.

When the cells attached, we passaged them upon confluence.

Cultures were passaged by 0.05% Trypsin/EDTA at a 1 3 or 1 4 ratio upon confluence.

Human fibroblasts were isolated from fetal liver and grown in Isocove's modified Dulbecco's medium containing 10% FBS, and passaged upon confluence [1].

Further passages were performed upon confluence.

Upon confluence, selected cells were passaged and serially cultured.

Upon confluence, the cell layer was scratched with a P-200 pipette tip.