HUVECs were grown to confluence on type I collagen-coated culture inserts with 0.4- μm pores (BD bioscience), treated with or without test samples for 18 h.
Immortalized SVCs stably expressing 7TFP were grown to confluence in type I collagen-coated 24-well plates in complete growth media, then treated with indicated concentrations of iCRT14 (or DMSO) in serum-free media for 24 hr.
Safb1-null MEFs also reached a higher density at confluence than wild type cells.
Briefly, HUVEC cells (Human Umbilical Vein Endothelial Cells) are grown as a monolayer to confluence on a type I collagen matrix layer poured onto a microporous membrane.
The San Blas Islands, or Kuna Yala Reserve, as they are also known, are a rare confluence of two types of paradise: tropical and cultural.
The technology of modern mass communication results from the confluence of many types of inventions and discoveries, some of which (the printing press, for instance) actually preceded the Industrial Revolution.
In a concert tradition that has taken hold in recent years, the days after Thanksgiving see a confluence of a certain type of rock band, the kind with, well, a certain level of melodic stamina: the kind that can play all night, it seems, and sometimes does.
We tested whether the spatial distribution of beds of preferred ammocoete habitat depends upon a specific set of geomorphic variables (field-measured slope, and geographic information system (GIS)- derived curvature, radius of curvature, presence of a confluence, and valley wall type).
Decrease in gene expression of markers of osteo/chondrogenic, myogenic, immune and vasculature cell lineages (Fig. 5) and increase in adipogenic markers indicate that the preadipocyte precursors, probably due to their active proliferation, become the preponderant cell type at confluence.
To ensure isolation between the rafts and the plated cells, the rafts were transferred to new dishes with adherent cultures of the same respective cell types (~70% confluence) at each change of media, every 2 days.
This summer, a perfect storm of a different type -- the confluence of environmental activism, scientific research and sentiment -- has called attention to this offbeat, largely forgotten chapter of Wallingford's past.
