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HBMECs were seeded on the inside of the insert and cells were grown to confluence to create a contact co-culture model (Allen and Bayraktutan, 2009; Mestre et al., 2011).
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Lawmakers on both sides are now lamenting the fiscal train wreck that many of them voted to create, a confluence of spending cuts and tax increases that the Federal Reserve chairman, Ben S. Bernanke, said Tuesday could send the economy into recession.
But it's worth noting that what likely merged to create the turbulent confluence that so retarded PC sales is in decline itself.
The chaotic confluence of the elements comes together to create a spontaneous interplay of light and shadow in Simon Heijden's latest installation, Shade.
On shallow rocky habitats, the confluence of some of these stressors helps to create one of the most degraded underwater communities in the Mediterranean – infralittoral barrens.
In our study, cells were seeded into six-well plates, grown to confluence and scratched with a p200 pipette tip to create a wound.
The blueprints are designed to create and share work within the Confluence environment.
Cells were grown until confluence, and a 20-p micropipette tip was used to create a linear scratch in the monolayer.
With the use of dedicated stents, we introduced an alternative technique to create an efficient and safe true confluence configuration (Figs. 2, 4).
Cells (5 × 10/well) were seeded in a 24-well plate and cultured to reach confluence and then scraped with a sterile micropipette tip to create a denuded zone (gap) with a constant width (T0).
Cell culture media was changed every 2 days until confluence, after which serum-free SmGM-2 media was used to create a nutrient-starved environment for 24 h.
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