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Human breast cancer cell lines were harvested at 80% confluence through trypsination.
In extra-cardiac Fontan, the systemic venous blood is directed to the main PA confluence through an extra-cardiac conduit.
As our data demonstrate, TPA-F HRBEC cultures re-entered the cell cycle within 3 days of TPA removal from the growth medium, and attained confluence through robust proliferation.
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Fig. 1 Computed tomography angiography shows that the vertical vein from pulmonary venous confluence goes through the diaphragm and drains into the portal vein, winding its way like a spiral.
First, we isolated CEFs from the developing face of control and ta embryos, plated the cells to confluence and scratched through the cellular monolayer.
RNA, DNA and proteins were extracted from frozen human bladder samples and from human bladder cancer cell lines grown to 70% confluence, by centrifugation through a cesium chloride density gradient.
A substantial amount of funding came about through the confluence of Rock Hudson and Elizabeth Taylor, initially, which is the celebrity aspect, and then through the gay community basically pressuring Congress.
Down to the Vasyugan confluence the river passes through the southern belt of the taiga, thereafter entering the middle belt.
Like Mr. Cunningham's artistry, Mr. Greenberg's resonates through its confluence of the random and the necessary; the continuous stream of motion in which no one moment is particularly important and each is beautiful; the almost magical quality of occasional formal symmetries.
Through a confluence of chance events -- ranging from mechanical glitches (the now infamous "hanging chad" throwing off automated ballot readers) to the zigzag trail of political and legal decisions that might have gone either way -- what the people of Florida really said may be not just uncertain but unknowable.
But through a confluence of events, Mr. Boehner and the rest of the anti-earmark crowd did get there; the impact of the decision by leaders of the House and the Senate to ban earmarks for at least the next two years is already being felt.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com