Because of this confluence, the rate and time components must be separated in order to make direct comparisons of substitution rates among taxa.
Analyses of long-term cultures showed that the use of APM did not affect cell proliferation as cell number at confluence and proliferation rate were similar whether primary cultures had been maintained with APM or FCS.
30 The neutral trend in mortality could be related to the confluence of lower rates of programme participation, the nature of participating patients being older and of lower socioeconomic status, improvements in secondary preventive care over time, and variation in risk factor control with some increasing and some decreasing.
NSLT infected with empty vector slowed their proliferation rate around confluence whereas SMDF-expressing cells, like those expressing Ras, kept proliferating reaching a higher cell density.
As cultures reach confluence and the proliferation rate decreases, steady, or dramatic increases in average lipofuscin levels are observed with maintenance or acceleration in the rate of lipofuscin accumulation, respectively.
Cell lines were subconfluent, and each cell line has been optimized in previous titration experiments so that cell type-specific optimal conditions for the seeding rate and confluence were obtained for the real-time measurement in these experiments.
Many urban planning experts see something other than a confluence of low interest rates, a tight housing market and developers with a gleam in their eyes.
But, in an unusual economic environment largely created by the confluence of low interest rates and poor fundamentals, they are concentrating their spending on a narrow universe of well-located office buildings with stable leases.
To study the contact inhibition of the cell lines expressing different levels of Arl2 we have evaluated their respective proliferation rates at confluence using phase contrast microscopy, MTT assays and flow cytometry analyses.
We note that at earlier times within each passage (i.e. when the cultured cells are proliferating fast) a greater percentage of nuclear Hes3 is observed than at later times as used here (i.e. when the cultures are reaching confluence and the proliferation rates decrease) (data not shown).
