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The growth rate of primary cell cultures varied considerably, with cells from the aorta, kidney and foetus growing to confluence quickly and requiring passage within 6 days.
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Briefly, cells were grown to 70% confluence, trypsinized quickly washed with soybean trypsin inhibitor, centrifuged, and resuspended in phenol red and serum-free DMEM (Gibco-Invitrogen).
Confluence was reached quickly as cell populations formed contiguous, flat monolayers (Fig. 3Aiii).
For seeding densities of 500 and 700 cells/mm2 (figure 9c d), the population levels quickly approach confluence.
When the cells reach 80% confluence, the media were quickly removed, and the dish was placed on top of dry ice.
As company CEO Dave Link sees, we are seeing this vast confluence of technology influences coming together very quickly.
Instead there has been a confluence of events – firstly house prices increased so quickly in Sydney and Melbourne that they reached a point where demand fell – because they were literally too expensive, especially in a period of flatlining household incomes.
The cells proliferated quickly, displaying obvious karyokinesis, after about 5 days, the cells confluence degree could reach 80 90%; then, the cells were digested by 0.25% trypsin and 0.02% EDTA.
It's a confluence.
This confluence pleased Gibbons.
The confluence of a giant market and cost-conscious consumers has forced them to squeeze component costs, make contract manufacturing more efficient and adopt technological innovations more quickly.
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