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Over four years of possible detection of emigration from the Isolated tributary (PIT tag antenna placed at the confluence), less than 0.1% of the Isolated population has been observed moving from the Isolated tributary to the WB and none have entered the Isolated tributary from the WB.
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All cells were harvested using trypsin/EDTA when confluence was less than 80%%.
SK-N-MC attach more slowly to the surface after splitting than SH-SY5Y and are sensitive to transfection if confluence is less than 75%.
Freshly isolated EBECs reached confluence in less than 5 days, in agreement with previous reports for equine [ 10, 11, 24] and human [ 19, 22, 23, 25] tracheobronchial epithelial cells.
When differentiation was induce before the cells had reached confluence, cells were less differentiated than under "classical conditions" and C/EBP-α was expressed at low level, that did not allowed quantification.
That confluence is made blessedly less hectic by Lincoln becoming a narrow one-way street, creating a small oasis in the city lined with businesses focused on serving the neighborhood as well as plenty of green spaces.
But my optimism is based less on confluence of political interests than it is the American people.
This observation can be explained by the fact that once the cells form confluence they are inhibited less than when they have not.
And it would never have revived itself without the more-or-less accidental confluence of some extraordinary people, on both sides of the Atlantic.
The confluence and contracting cultures are less significant in the sparse grid.
Balb/MK2 keratinocytes were exposed at less than 50% confluence to 10 mJ/cm UVB with a calibrated UVB lamp as previously described.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com