HBMECs were seeded on the inside of the insert and cells were grown to confluence to create a contact co-culture model (Allen and Bayraktutan, 2009; Mestre et al., 2011).
Along with My Father's Place, which opened on Memorial Day in 1971 with a concert by Richie Havens, a confluence of entities created a scene that would influence music for decades to come.
A confluence of circumstances created the sea change for McKnight in the mid-2000s, including finding his half brother in England -- the other son whom his father, a pilot in World War II, had told him about briefly.
HMVEC-L were plated to confluence and wounds created in the monolayer by scraping the plate with a pipette tip.
In order to address the misperceptions, fear, suspicion and lack of trust this confluence of issues created, UNICEF partnered with key opinion makers, professionals and influential figures within the affected communities.
A confluence of factors has created a more favorable environment for developers.
"My insistence on having a speaker from Hudson County probably created a confluence of things that made it possible for Albio," Mr. Menendez said.
Huxley's Englishness is, in a sense, self-evident, his Eton-Balliol-Bloomsbury formation the confluence of privileges which created the voice described by Robert Craft as a 'lambent, culture-saturated purr', the sheer, blithe opinionation that allowed him to come up with formulas like 'Penang has a certain Sicilian air', but he spent a third of his life based in America.
After the cells reached semi-confluence, a wound was created in the cell monolayer using a pipette tip.
