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In our study we seeded the cell cultures overnight after the cells had reached 80% confluence (i.e. proliferative phase) to ensure the cells were expressing the maximum amount of σ2 receptors in the cells, also to optimise signal-to-noise ratio in cell uptake studies.
Cells were subcultured to a fresh culture dish when growth reached 70 90% confluence, i.e. every 2 3 days.
The highest DON level (4000 ng/mL) resulted in a TEER-decrease even below the level of cellular confluence, i.e. 1 kOhm, after 72 hours exposure.
The N/C ratio decreased to 1/1 when cells reached confluence, i.e. when most cells were in the G0/G1 phase of the cell cycle, suggesting that cells need a reduced nuclear environment to proliferate.
All experiments showed optimal confluence (i.e., > 70%) except one, which displayed a confluence of about 50 60%.
Cells were sub cultured to a fresh culture vessel when growth reached 70to90%0% confluence (i.e., every 3 to 5 days) and were incubated at 37°C in a humidified atmosphere of 95%air 5%% CO2.
Similar(54)
In particular, the degree of hierarchical organization of the drainage network is assessed through the hierarchical anomaly density, a measure of the number of anomalous confluences (i.e. channels of u order not flowing in channels of order u + 1) in drainage basins.
Five days past-confluence I-VEGF/VEGFf binding was conducted with isolated ECM.
In one of those strange confluences, I was, at the same time, writing a novel about a woman who had just given birth.
But doing this with women can be complex for men -- for me -- as complex as the confluence of emotions I feel when I'm alone with them in my office.
When I think of the bakery where I worked, I think of the yeast and butter, tile and apples, a confluence so distinct I can taste the pain au levain in my head and I can smell the cool street air that came in with the delivery man.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com