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Conflicting alignments can result in ambiguous placement of an experimental map on a database of in silico maps, but condition (a) could be relaxed in some cases, for example, when experimental maps are known to overlap multiple in silico maps in the same region.
Because the set of Sanger ESTs was not exhaustive, we attempted to identify well-assembled ESTs from the subset with conflicting alignments by comparing them to the NR protein database to determine whether there is a known protein that covered the full length of the Illumina EST.
Because of these severely broken and sometimes conflicting alignments, no further efforts could be made to find a unified order for these remaining central heterochromatic RH and DM sequences.> -wrap-foot> (a) Percent phase 1 sequence recovered in phase 0 sequence.
If any pairwise overlap alignments conflict with the current multiple alignment graph, corrective algorithms use the conflicting alignments to either ignore the new pairwise overlap (if the graph is more consistent) or to correct the constructed multiple alignment (if the new pairwise overlap identifies a misalignment in the graph).
The Hybrid Scaffold program only generates hybrid in silico/consensus genome map contigs, and therefore super scaffolds, if no conflicts (e.g. negative gap lengths or otherwise conflicting alignments) are indicated in the alignment of in silico and consensus genome maps.
In this conservative approach, all conflicting alignments are excluded from the hybrid scaffold genome map and flagged for further evaluation at the sequence level.
Similar(53)
Therefore, Hybrid Scaffold would only report the conflicting alignment.
Since this step is performed based on the overlap of sequence intervals without explicitly considering the sequence information at all, TRACKER is very fast as long as the number of conflicting pairwise alignments remains small.
A sub-map alignment π1 is said to be conflicting with another alignment π2 if either: the sub-map of π 1 overlaps the sub-map of π 2; or π 1 aligns to the same in silico map as π 2, but in a different location or strand.
Current methods assess structural alignment quality by constructing a scoring function that attempts to balance conflicting criteria, mainly alignment coverage and fidelity of structures under superposition.
To generate compatible sequences alignments for coalescent analyses, the conflicting sites in each alignment were manually removed [ 46].
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